201 The Assessment of Ex-Vivo Human Pancreata Using Magnetic Resonance Imaging
Monday November 16, 2015 from 07:00 to 08:00
Room 110

Hussein A Khambalia, United Kingdom

Transplant Registrar

Department of Transplantation

Manchester Royal Infirmary


The Assessment of Ex-Vivo Human Pancreata Using Magnetic Resonance Imaging

Hussein Khambalia1, Sha Zhao2, David van Dellen1, Angela Summers1, Zia Moinuddin1, Giuseppe Giuffrida1, Petros Yiannoullou1, Geoffrey Parker2, Titus Augustine1.

1Department of Transplantation, Manchester Royal Infirmary, Manchester, United Kingdom; 2The Centre for Imaging Sciences, University of Manchester, Manchester, United Kingdom

Introduction: Outcomes following pancreas transplantation are closely linked with the pre-operative condition of the organ. Increased fat and fibrosis content are recognised as leading to poorer outcomes following implant. However, current assessment of the allograft prior to implantation is entirely subjective, leading to inevitable variation between surgeons and potential underutilisation of suitable organs.
Magnetic Resonance Imaging (MRI) provides the ability of viewing tissues non-invasively, leading to a possible method of critically analysing organs and therefore providing an objective method of assessing pancreata prior to implantation.
We aimed to conduct a preliminary study to assess the feasibility of conducting MRI on ex-vivo human pancreata and explore the potential advantages of MRI assessment in the evaluation of the organ prior to transplantation.
Methods: Those pancreata deemed unsuitable for transplantation underwent MRI on a Philips Achieva 3.0T system. Pancreata were scanned in 3 bags containing UW preservation solution, surrounded by a further bag of ice. MRI protocols were developed to acquire high-resolution 3D volume images of water and fat, and of T1 and T2 weightings within the specimen at 4oC.
Results: Images from water and fat protocols at equivalent sections of an ex-vivo pancreas, deemed unsuitable to transplant are presented (Figure 1 and b respectively), indicating excellent differentiation and identification of fat and “other” tissue within the specimen. T1 and T2 weighted images of pancreata also offer potential protocols for objectively assessing the condition of the organ.
Conclusions: MRI appears to be a feasible method of assessing ex-vivo human pancreata with respect to fat content. Potential utilisation of this technique may rest in the ability to quantify fat content within the specimen, but this requires further analysis. Based upon the calculated protocols used in this study and the results presented, it is possible that in the future, MRI could be a valuable tool in aiding clinicians to objectively assess suitability of pancreata prior to implantation.

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