Seoul National University Hospital
Effect and safety of anti-CD40 antibody on the survival of corneal xenotransplantation
Jaeyoung Kim1,2,3, Dong Hyun Kim1,2,3, Hyuk Jin Choi1,2,3, Hyun Ju Lee2, Ji Hyun Kim2, Hee Jung Kang4, Chung-Gyu Park3,5, Won Ryang Wee1,2,3, Mee Kum Kim1,2,3.
1Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea; 2Laboratory of Ocular Regenerative Medicine and Immunology, Seoul Artificial Eye Center, Seoul National University Hospital Biomedical Research Institute, Seoul, Korea; 3Xenotransplantation Research Center, Seoul National University College Of Medicine and Clinical Research Institute, Seoul, Korea; 4Department of Laboratory Medicine, Hallym University College of Medicine, Anyang, Gyeonggi-do, Korea; 5Department of Microbiology and Immunology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
To investigate the effect and safety of systemically administered anti-CD40 antibody on the survival of deep lamellar porcine corneal grafts and the immunologic profile of the recipients in pig-to-rhesus corneal transplantation model. Five Chinese rhesus macaques underwent corneal transplantation using clinically acceptable sized (7.5 mm in diameter) porcine corneal grafts. Intravenous anti-CD40 antibody and immunoglobulin were administered as programmed schedule. Anti-CD 40 antibody-based immunosuppressive treatment achieved the successful survival of xenocorneal grafts (> 9 months, > 9 months, > 6 months, > 5months) in 4 primates. One primate which expired accidentally at 2 months due to asphyxia after anesthesia maintained transparent graft until death. Central or effector CD8+ T or central memory CD4+ T cells in Anti-CD40 antibody treated primates were comparable to those in Anti-CD154 antibody-treated primates, while effector memory CD4+ T cells tend to be higher than those in Anti-CD154 antibody-treated primates. Anti alpha-Gal Ig G and Ig M levels of the blood in Anti-CD40 antibody treated primates were lower than in Anti-CD154 antibody-treated primates, while complement responses of aqueous humor were increased over time after transplantation. No Porcine endogenous retrovirus and Cytomegalovirus were detected in Anti-CD40 antibody treated primates. General condition, liver function and renal function test are within normal range in all Anti-CD40 antibody treated primates. The effect and safety of Anti-CD40 antibody treatment on corneal deep lamellar xenotransplantation in primates appears to be comparable to the effect and safety of Anti-CD154 antibody treatment on corneal xenotransplantation.
The Korea Healthcare Technology R&D Project, Ministry for Health & Welfare, Korea (HI13C0954).
07:00 - 08:00
|Cell & Tissue Xenotransplantation: Tolerance||Effect and safety of anti-CD40 antibody on the survival of corneal xenotransplantation||Room 109|